Epididymal sperm harvest












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The wide range in pregnancy rates is due to factors such as health status of the stallion prior to castration or euthanasia, interval from castration to harvest of epididymal spermatozoa, post-thaw motility, and reproductive status of mares that are bred. Each ductus deferens was identified and ligated with surgical suture to prevent loss of spermatozoa during collection and transport. A straw from each type of extender was thawed and evaluated the next day. The extended semen was transferred into 0. Pregnancy rates with frozen epididymal semen have ranged from 0 percent to 66 percent.

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Pregnancy Outcomes Using Stallion Epididymal Sperm Stored

Each testes, epididymis, and associated ductus deferens were removed as an intact unit. The ductus deferens and epididymis were flushed with seminal plasma previously harvested from other stallions followed by a second flush with semen extender Fig. During spermatogenesis, primary sperm cells of the male undergo meiosis and eventually form spermatozoa containing a haploid number of chromosomes. Flushing seminal plasma through the ductus deferens white arrow and out the epididymal tail grey arrow to collect spermatozoa into a glass flask. The epididymides are the primary sperm storage sites, containing an average of 8 billion to 10 billion spermatozoa or more between the two combined epididymides. Intracytoplasmic sperm injection ICSI may also be used with epididymal semen to conserve spermatozoa, as the procedure requires injection of only one spermatozoon into an oocyte.

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Sperm retrieval for ivf

The aims of this study were to. Signalment and History. Millions of additional spermatozoa are contained within the ductus deferens vas deferens , the tubular tract that connects the tail of the epididymis with the pelvic urethra Fig. Take Home Message It is possible to preserve genetic material from stallions that sustain a catastrophic injury by harvesting and freezing epididymal spermatozoa. Results of post-thaw motility for the epididymal semen collected and frozen from the injured stallion. Semen frozen in MFR-5 had lower post-thaw motility, and advanced assisted reproduction techniques may be required to generate pregnancies using that cryopreserved semen. Discussion Point Approximately 10 to 15 billion sperm can be harvested from the paired epididymides of most stallions for cryopreservation.

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Description: Pregnancy rates with frozen epididymal semen have ranged from 0 percent to 66 percent. Semen frozen in MFR-5 had lower post-thaw motility, and advanced assisted reproduction techniques may be required to generate pregnancies using that cryopreserved semen. The pregnancy rate using epididymal sperm stored for 24 hours before harvest is consistent with that of previous reports. Straws of frozen semen were transferred into a tank of liquid nitrogen for storage Fig. Discussion Point Approximately 10 to 15 billion sperm can be harvested from the paired epididymides of most stallions for cryopreservation. Thirteen mares were bred on one cycle with cryopreserved epididymal sperm stored for 24 hours before harvest, and 10 of those 13 mares were also bred on a previous or subsequent cycle with samples from the same stallion that had been stored for 48 hours before harvest. No attempt was made to separate the epididymis from the testis. Ultimately, 92 straws of epididymal semen were frozen. Frozen epididymal semen yields an equivalent pregnancy rate to frozen ejaculated semen when using ICSI.
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